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. 2022 May 25;20(9):1379–1390. doi: 10.1158/1541-7786.MCR-22-0165

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©2022 The Authors; Published by the American Association for Cancer Research

This open access article is distributed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license.

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Figure 4. Targeting MUC1-C suppresses ASCL1, NEUROD1, and YAP1 expression. A, Lysates from H69/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. B, Lysates from H69/CsgRNA, H69/MUC1sgRNA#1, and H69/MUC1sgRNA#2 cells were immunoblotted with antibodies against the indicated proteins. C, Lysates from DMS53/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. D and E. Lysates from H69/tet-MYCshRNA (D) and DMS53/tet-MYCshRNA (E) cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. F and G. Lysates from COR-L279/tet-MUC1shRNA (F) and H526/tet-MUC1shRNA (G) cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. — Targeting MUC1-C suppresses ASCL1, NEUROD1, and YAP1 expression. A, Lysates from H69/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. B, Lysates from H69/CsgRNA, H69/MUC1sgRNA#1, and H69/MUC1sgRNA#2 cells were immunoblotted with antibodies against the indicated proteins. C, Lysates from DMS53/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. D and E, Lysates from H69/tet-MYCshRNA (D) and DMS53/tet-MYCshRNA (E) cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. F and G, Lysates from COR-L279/tet-MUC1shRNA (F) and H526/tet-MUC1shRNA (G) cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins.