Fig. 2.

Dynamic distribution of viruses in CV-A10-infected rhesus macaques. Rhesus macaques in RI and DI groups were infected with CV-A10 (10⁵ CCID₅₀/monkey) via respiratory tract or digestive tract respectively. The three rhesus monkeys in GC group were not treated. The viral loads in blood and swab samples were monitored to evaluate viral replication kinetics in rhesus macaques at the scale of copies/200 ​μL or copies/100 ​mg qRT-PCR based on the TaqMan probe method was performed after extracting viral RNA to determine the CV-A10 RNA load at specific time points. A Detection of viral RNA in blood. B Detection of viral RNA in pharyngeal samples. C Detection of viral RNA in fecal samples. D On day 7 following infection, the homology of one randomly chosen RT-PCR product from rhesus monkey blood, a pharyngeal swab sample, and feces was compared to the reference sequence. The viral copy number was quantified based on in vitro synthesized 5′UTR protein RNA by the formula [(micrograms of RNA/μL)/(molecular weight)] ​× ​Avogadro's number ​= ​viral copy number/μL. At each time point, the values are represented as the average of two measurements.