Fig. 3.

Etiological and pathologic observation of macaque infected CV-A10 via digestive tract. A Changes in body temperature and body weight at 0–10 ​d.p.i.; B viral load in blood at 0–10 ​d.p.i.; C viral load in throat swab samples at 0–10 ​d.p.i.; D dynamic changes in viral load in feces at 0–10 ​d.p.i.; E viral copy number in tissues from 24 different anatomical sites at 0–10 ​d.p.i., showing nucleic acid positivity in the brain, visceral, immune, and intestinal tissues, clearly indicating that the multiple tissues were subjected to viral infection. F H-E staining of lung and heart tissues. G H-E staining of liver and spleen tissues. H immunohistochemical staining analysis of CV-A10 in lung, liver and pancreas tissues. Red arrows represent inflammatory granulomas, yellow arrows represent foam-like cells, green arrows represent lymphocytic infiltrates, and black arrows represent inflammatory cell infiltrates. I immunohistochemical staining analysis of CV-A10 in heart, spleen and kidney tissues. The arrow shows the expression of CVA10-positive antigen. The black scale bar indicates 50 ​μm.