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. 2022 Aug 19;10:918690. doi: 10.3389/fbioe.2022.918690

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Copyright © 2022 Potere, Belgio, Croci, Tabano, Petrini, Dubini, Boschetti and Mantero.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Microscope images were acquired on the sample section. The red line indicates the section plane on which the acquisition took place. The analysis of cell viability in the 3D bioprinted constructs was performed using a Live/Dead viability/Cytotoxicity kit (Invitrogen, United States). (A) Fluorescence images were taken at 1 day, 5 days, and 10 days to evaluate the live (green) and dead (red) staining cells in the printed constructs with microscopy Eclipse Ti2-E (Nikon). Three random views from each sample were photographed and analyzed using Fiji ImageJ software. From these images, we can say that the developed bioink is biocompatible and cytocompatible. (B) Images of the cell infiltration were captured at 15 and 20 days of culture. Comparing the images of different time points we can appreciate a cell growth into the 3D Bioprinted construct.