Figure 8.

PPI analysis of DEGs. (A) PPI network. The protein interaction relationships of targeted DEGs were determined based on the genome-wide PPI network of apple (GDDH13). The predicted protein associations were determined from the STRING database, and the PPI was built based on experimental, text mining, database, and co-expression evidence. The size of the node is the parameter of the connectivity of the network. Multiple copies of identical genes are shown as a single node. For example, the large green circle at the bottom of the figure represents five sequences predicted as the TOR gene in the RNA-seq data, including MD05G1107800, MD00G1031100, MD10G1086500, MD10G1112000, and MD15G1045400. They coincided completely in the PPI network. The top small green circle represents novel.4829 in the RNA-seq data. (B) Interaction analysis between predicted TOR and proteins in the JA pathway. The 618 amino acids of the MD05G1107800 N-terminal and 400 amino acids of the MD10G1086500 N-terminal were used to construct BD vectors. JMT, JAZ10, and NPR3 were separately cloned into AD vectors. The recombined BD and AD vectors were then co-transformed into yeast and grew on different media. T, L, H, and A are short for Trp, Leu, His, and Ade. The line connecting TOR and NPR3 was colored red after the interaction was confirmed in yeast. The blue line connecting TOR and JMT and the blue line connecting TOR and JAZ10 were added to the PPI network according to the results in yeast.