Figure 2.

Activity of c/EBPα, SOX9, and TGFβ signaling during development of hepatocytes and cholangiocytes from hepatoblasts

(A) Experimentally observed adult hepatocyte signature, c/EBPα regulon activity and TGFβ signaling activity in a population of single cells of hepatocytes/hepatoblasts at different developmental times (GSE90047). ∗ represents a statistically significant difference in activity levels (Students’ two-tailed t test; pvalue<0.05) while comparing E10.5 and E17.5.

(B) Experimentally observed adult biliary signature, SOX9 regulon activity and TGFβ signaling activity in a single-cell RNA-seq dataset containing mature/immature cholangiocytes with developmental time (GSE90047). ∗ represents a statistically significant difference in activity/expression levels (Students’ two tailed t-test; pvalue<= 0.05); ns indicated non-significant.

(C) Trajectory analysis of hepatocytes and cholangiocytes as they form from progenitor cells (colored by c/EBPαand SOX9 regulons showing their specificity to hepatocyte and cholangiocyte fate respectively).

(D) Cell entropy values seen to be the maximum in progenitor cells and decreases as cells enter more mature states.