Table 1.

Summary of commercial nucleic acid extraction buffers used in this study.

Extraction Buffer (EB)	Kit	Vendor	Guanidine Thiocyanate content	Sample:EB Ratio	Additional Components Added	Sample/Buffer Incubation Time (Kit)
Viral RNA Buffer	Quick-RNA Viral Kit	Zymo Research	≤6 M (70 %)	1:3	0.5% β-ME added	Not Specified
Binding Buffer	High Pure Viral Nucleic Acid Kit	Roche Diagnostics	None (6 M Guanidine HCl)	1:1	Proteinase K	Proteinase K +10 min
MagMAX Lysis/Binding Concentrate	MagMAX Pathogen RNA/DNA Kit MagMAX-96 Viral Isolation RNA Kit	Applied Biosystems	4.65–6.77M (55–80 %)	1:2	None	Not Specified
Buffer AVL	QIAamp Viral RNA Mini kit	Qiagen	4.2–6 M (50–70 %)	1:4	Ethanol added (44 % final)	10 min
UNEX	N/A	VWR/Microbiologics	4.1 M (48 %)	1:1	Ethanol added (50 % final)	10 min
Trizol LS	N/A	Invitrogen	1.3–3.4 M (15–40 %)	1:1	None	Not Specified