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. Author manuscript; available in PMC: 2022 Sep 2.
Published in final edited form as: J Virol Methods. 2021 Aug 9;297:114262. doi: 10.1016/j.jviromet.2021.114262

Table 5.

Minimum incubation time for WPV1 inactivation.

Incubation Time
Extraction Buffer Sample:Buffer Ratio Additional Components 5 min 10 min 15 min 20 min
Trizol LS 1:3 None Below LOD NT NT NT
Viral RNA Buffer 1:3 0.5% β-mercaptoethanol Below LOD NT NT NT
Buffer AVL 1:4 Ethanol (44 % final) Below LOD NT NT NT
UNEX 1:1 Ethanol (50 % final) 0.7 ± 0.3 Below LOD NT NT
MagMAX Lysis/Binding Concentrate 1:2 None 0.6 ± 0.06 0.5 ± 0.06 NT NT
4 M Guanidine Thiocyanate+ 20 % Ethanol 1:4 Ethanol (20 % final) 1 ± 0.3 0.9 ± 0.3 1.1 ± 0.1 0.8 ± 0.1

WPV1 isolate was incubated with extraction buffers Buffer AVL with ethanol, Trizol LS, UNEX, Viral RNA Buffer, MagMAX Lysis/Binding Concentrate, or 4 M guanidine thiocyanate with 20 % ethanol for the times indicated below. Samples were concentrated and resulting viral titers determined by CCID50 assay. LOD, Limit of detection (0.5 log10 CCID50/0.1 mL). NT, Not Tested.