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. 2022 Aug 23;56:102446. doi: 10.1016/j.redox.2022.102446

Fig. 4.

Fig. 4

LDHA overexpression improved cardiac function after MI in adult mice.

(A) Schematic of the MI experiments in adult mouse hearts injected with AAV9. Echo, echocardiography. (B) Ki67 staining in adult mouse MI model hearts at 21 days post-MI (866 CMs from 12 images of 6 mice in cTnT-NC and 1003 CMs from 12 images of 6 mice in cTnT-LDHA groups). (C) pH3 staining in adult mouse MI model hearts at 21 days post-MI (1459 CMs from 24 images of 6 mice in cTnT-NC and 1825 CMs from 24 images of 6 mice in cTnT-LDHA groups). (D) Aurora B staining in adult mouse MI model hearts at 21 days post-MI (4239 CMs from 6 mice in cTnT-NC and 3504 CMs from 6 mice in cTnT-LDHA groups). (E) WGA staining of left ventricle heart sections from adult MI model hearts at 21 days post-MI (219 CMs from 15 images of 5 mice in cTnT-NC and 212 CMs from 15 images of 5 mice in cTnT-LDHA groups). (F) Masson straining of heart sections in adult mice at 49 days post-MI. n = 8 mice. (I) Cardiac function was analyzed using echocardiography at pre-MI and 7, 21 and 49 days post-MI. n = 10 mice. Bars = 20 μm in B-E and 1 mm in F. Statistical significance was calculated using an unpaired t-test in B-D, nonparametric Mann-Whitney U test in E, one-way ANOVA in F and two-way ANOVA in H; *P < 0.05.