Table 3.
The primer sequences used for qPCR validation.
| Primers | Sequences (5’-3’) |
|---|---|
| mRNA-seq | |
| TLR1-F | CCAGGGTCGCAGAGTCCTATC |
| TLR1-R | GCCAGCCAAGTTCAGTTTCGT |
| TLR2-F | AGGGTTCAGAAGGGTTGCTAT |
| TLR2-R | CAGGAAGGAAGTCCCGTTTGT |
| TLR3-F | CTGGCTTACTACAACCACCCC |
| TLR3-R | CAAACTCCCTGCCCTCTTCA |
| TLR7-F | AAGGTCATAGGATTTGGAGCA |
| TLR7-R | AGGGAGAAACTGACGGCTTAA |
| TLR8-F | CGCTTGGACAGTGGGTTTCTT |
| TLR8-R | GAACTTCGTCCTTCTGGTTCG |
| IRF3-F | CTGGCTTACTACAACCACCCC |
| IRF3-R | CAAACTCCCTGCCCTCTTCA |
| MRC1- F | CAGACGGGAAGACCTGGTTCG |
| MRC1-R | ATCCAGACTGATTCATAGCGT |
| LRP1-F | CATTGGCTATTATGGGAGAAA |
| LRP1-R | TTGTTTCGCAAAATCTTCCAG |
| LDHA-F | ATCATCCCGAACATCGTCAAG |
| LDHA-R | GGTGGCGGAAACGGGCAGAGT |
| JUN-F | CTCTTTTCTGTCGGCTTACGG |
| JUN-R | CCGCTTGGTACGGGTCTGTCT |
| FOS-F | CTGACAGCATCAAGTGCCTCC |
| FOS-R | GCAGAGTTATGAGCCTTGGAT |
| CCCX1- F | TGGTCCAGCAACCTAACCTTC |
| CCCX1-R | AGGGGAGGCAGTGGTTGTGAT |
| CCCX3-F | AAACATCACCGCTCCCATCAT |
| CCCX3-R | AACACCCAGTCCTGCTTCCAG |
| C9-F | GTCTTGTCAGGGATCAGTGGG |
| C9-R | CTGCCTTGCTCATTGCTATCC |
| TRAF3IP3-F | TTTTCCTTCTTCTTCTCGCTGTG |
| TRAF3IP3-R | CATGAGATAATCTTCGATACG |
| MAP3K5-F | CGAGACCAATGAAAATGGCGAC |
| MAP3K5-R | ACCATCCTGACTGACAGAGCC |
| C-lectin-F | ATCGCATAACAGAGCCAGAC |
| C-lectin-R | CAGGGAACATCACTCCAAAC |
| C7-F | GCTGGAGAAAGGTGAAACGCCGT |
| C7-R | CCAGTTGTCGTATTTCTCTCCGTT |
| C3-F | CCTCAACAAGTTTGCTTCC |
| C3-R | TTATAGTAGCCTGAGTTGATCCGTA |
| SRECI-F | GCAGGACTTGAACGGCTCG |
| SRECI-R | CATAATGGCTGTCTTTTGCTGC |
| SRECII-F | GGGGAGGTCGGCATTTGT |
| SRECII-R | ATTCACCTCTGGCACGCTCTT |
| CD163L1-F | GGACAACCGAAAAGTCTAATT |
| CD163L1-R | CATAGGCTGGGTCATAGTCGG |
| IL-6-F | CAATCCCAGCACCTTCCAC |
| IL-6-R | CCTGACAGCCAGACTTCCTCT |
| PITX3-F | AGGGAAGAACAAGAACAAAACCTG |
| PITX3-R | TTACATCCCTGGTCGTGCTG |
| BPI-F | GACACCACATGACAAAGGCAC |
| BPI-R | ATGTTAAATCCTTGCACCCTCCA |
| EF-1α-F | GGTCGTCACCTTCGCTCCAT |
| EF-1α-R | TCCCTTGGGTGGGTCATTCT |
| miRNA-seq | |
| miR-126-y | TCGTACCGTGAGTAATAATGCA |
| miR-144-y | GCGGGAGTATAGATGATGTAC |
| miR-0459-5p | AAGCACCCCTAGTCGTGAGA |
| miR-0088-3p | CTCAGACTTAGGAAAACTTGC |
| miR-149-x | GCTCCGTGTCTTCACTCCA |
| miR-217-x | GGGGTACTGCATCAGGAACT |
| miR-0053-5p | TTCCGACCATGTTAGCACCA |
| miR-497-x | GGGGCACTGTGGTTTGTAAA |
| miR-7133-y | GGGTTTGATACACAGCACAATA |
| miR-142-x | GGGCATAAAGTAGAAAGCACTA |
| miR-499-x | GGGTTAAGACTTGTAGTGATG |
| miR-0440-5p | CAGGTCTCAGGTCTTAGGTC |
| miR-0468-5p | TGGAAGGCTGAGACACGAC |
| U6-F | U6 Forward Primer (Takara) |
| U6-R | U6 Reverse Primer (Takara) |
| Universal reverse primer | mRQ 3’ Primer (Takara) |