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FliM and FliN synthesis in minicells. Autoradiogram (12-h exposure) of 35S-labeled proteins synthesized in minicells containing plasmids. Lanes: 1, pLM2297 (fliMshort); 2, pLM2296 (fliM+); 3, pLM1877 (vector); 4, pLM2294 (fliM+N+); 5, pLM2297 (fliMshort). Arrows indicate polypeptides encoded by the fli genes and a vector-encoded product. The resolving gel contained 10.5% acrylamide. FliMs, the product of truncated fliMshort.
