TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or description | Source and/or parent; reference |
|---|---|---|
| Strains | ||
| V. parahaemolyticus | ||
| BB22 | Wild type | R. Belas; 4 |
| LM1017 | lfgE313::lux | BB22; 44 |
| LM4170 | motX118 lfgE313::lux | LM1017; 40 |
| LM4171 | motY141::mini-Mu lac lfgE313::lux | LM1017; 39 |
| LM4262 | motX1699::TnphoA | BB22; 40 |
| LM4289 | motY1719::TnphoA lfgE313::lux | LM1017; 39 |
| LM4474 | motY141::mini-Mu lac | LM4171; this work |
| LM4652 | motA2::TnphoA | BB22; this work |
| LM4653 | motA3::TnphoA | BB22; this work |
| LM4654 | motB2::TnphoA | BB22; this work |
| LM4655 | motA1::TnphoA | BB22; this work |
| LM4656 | motB1::TnphoA | BB22; this work |
| LM4657 | motA2::TnphoA lfgE313::lux | LM1017; this work |
| LM4658 | motA3::TnphoA lfgE313::lux | LM1017; this work |
| LM4659 | motB2::TnphoA lfgE313::lux | LM1017; this work |
| LM4660 | motA1::TnphoA lfgE313::lux | LM1017; this work |
| LM4661 | motB1::TnphoA lfgE313::lux | LM1017; this work |
| LM4806 | fliG1::TnlacZ/in | BB22; this work |
| LM4807 | fliG1::TnlacZ/in lfgE313::lux | LM1017; this work |
| LM4808 | fliG2::TnlacZ/in | BB22; this work |
| LM4809 | fliG2::TnlacZ/in lfgE313::lux | LM1017; this work |
| LM4810 | fliG3::TnlacZ/in | BB22; this work |
| LM4811 | fliG3::TnlacZ/in lfgE313::lux | LM1017; this work |
| LM4812 | fliN1::TnlacZ/in lfgE313::lux | LM1017; this work |
| LM4813 | fliM2::TnlacZ/in | BB22; this work |
| LM4814 | fliM2::TnlacZ/in lfgE313::lux | LM1017; this work |
| LM4815 | fliM1::TnlacZ/in lfgE313::lux | LM1017; this work |
| LM4889 | fliM1::TnlacZ/in | BB22; this work |
| LM4890 | fliN1::TnlacZ/in | BB22; this work |
| E. coli | ||
| DFB9 | thr(Am)1 leuB6 his4 metF(Am)159 thi1 rpsL136 Sm(R) lacY1 ara14 xyl5 mtl1 tsx78 tonA31 eda50 | D. Blair |
| DFB210 | ΔmotAB | DFB9, D. Blair |
| DFB223 | ΔfliN (in frame) | DFB9, D. Blair |
| DFB225 | ΔfliG (in frame) | DFB9, D. Blair |
| DFB228 | ΔfliM (in frame) | DFB9, D. Blair |
| DFB232 | ΔfliMN (in frame) | DFB9, D. Blair |
| Plasmids | ||
| pBBR1MCS | Camr; broad-host-range vector | M. Kovach; 27 |
| pLAFRII | Tetr; broad-host-range cosmid vector | 16 |
| pMMB66EH | Apr; broad-host-range expression vector carrying lacIq and Ptac | 17 |
| pRK415 | Tetr; broad-host-range vector | 24 |
| pUCGM | Genr | H. Schweizer; 49 |
| pLM1778 | Tetr; pLAFRII-derived cosmid carrying lfgE locus | This work |
| pLM1796 | Tetr; pLAFRII-derived cosmid carrying lafTU locus | 44 |
| pLM1877 | Genr Apr; broad-host-range expression vector | pMMB66EH; this work |
| pLM2058 | Tetr;pLAFRII-derived cosmid carrying motAB locus | 22 |
| pLM2047 | Tetr; pLAFRII-derived cosmid carrying fliF locus | This work |
| pLM2192 | Tetr; fliG+H+ | pRK415 and pLM2047; this work |
| pLM2293 | Camr; fliL+M+N+O+P+ | pBBR1MCS and LM2047; this work |
| pLM2294 | Genr; fliM+N+ | pLM1877 and PCR product; this work |
| pLM2296 | Genr; fliM+ | pLM1877 and PCR product; this work |
| pLM2297 | Genr; fliMshort | pLM1877 and PCR product; this work |