TABLE 2.
Insertion mutations in motor and switch genes
| Allele | Comments | Location of insertionb
|
|
|---|---|---|---|
| bp | aa | ||
| motA1::TnphoA | 576 | 58 | |
| motA2::TnphoA | 627 | 75 | |
| motA3::TnphoA | 1094 | 230 | |
| motB1::TnphoA | 2093 | 304 | |
| motB2::TnphoA | Blue fusiona, partial function | 2103 | 308 |
| fliG1::TnlacZ/in | Blue fusion | 2860 | 228 |
| fliG2::TnlacZ/in | Blue fusion | 2878 | 234 |
| fliG3::TnlacZ/in | 2536 | 120 | |
| fliM1::TnlacZ/in | 8613 | 23 | |
| fliM2::TnlacZ/in | Blue fusion | 9391 | 83 |
| fliN1::TnlacZ/in | Blue fusion | 9740 | 33 |
a
Blue fusion, insertion produced an active alkaline phosphatase or β-galactosidase fusion protein that was capable of converting the color of a chromogenic substrate to blue.
b
Location of insertion is given according to the base pairs (bp) on the physical map presented in Fig. 1, which corresponds to the sequence deposited in GenBank, and to the amino acid codon (aa) perturbed by the position of the transposon. The predicted total numbers of amino acids in the polypeptides encoded by each gene are 253 (motA), 316 (motB), 351 (fliG), 248, (fliM), and 136 (fliN).