FIG. 1.

Physical map of the S. epidermidis chromosomal region containing the tagF locus. The tagF gene is represented by the thick black arrow. Sequence analysis of the 3′ end of the 5.5-kb fragment in pBCP55 revealed the presence of an ORF which shares significant homology (P = 4 × e⁻²³) with the E. coli lctP gene encoding l-lactate permease (11). The partially sequenced genes pal1, tpgX, and lctP are represented by unshaded bars; the thin arrows above indicate the direction of their transcription. Regions of DNA cloned into pGN333, pGN23, pBCP55, pGDH3, pBH17, and pBN23 are represented by black bars. The position of the 260-bp tagF PCR product generated with the TP3 and TP4 primers is indicated with an asterisk. Reference restriction sites are indicated as follows: P, PstI; N, NsiI; H, HindIII; E, EcoRV; C, ClaI; A, AatII. Dotted lines mark the unsequenced region, which is not drawn to scale.