FIG. 3.
Complementation analysis of B. subtilis 1A486 with the S. epidermidis tagF gene. Strains were grown for 16 h at 42°C on LA plates containing chloramphenicol (5 μg ml−1). (A) Photomicrograph of strain 1A486(pHPS9) exhibiting the coccoidal morphology characteristic of the TagF1 (RodC1) phenotype. (B) Photomicrograph of strain 1A486(pFC10) showing the restoration of the wild-type rod-shaped morphology by complementation with the S. epidermidis tagF gene. (C) Comparison of growth of strains 1A486(pFCTH3) and 1A486(pFC10). (D) Comparison of growth of strains BSS40 and 1A486(pFC10).