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. 2022 Sep 2;13:440. doi: 10.1186/s13287-022-03135-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Karyotype, copy number variation (CNV) and precision of the CRISPR-Cas9 mediated NIPBL c.5483G > A correction in hiPSC-cl1. A Karyotype analysis conducted by carrying out a shallow Whole Genome Sequencing (sWGS) in hiPSCs-c.5483G > A. B Karyotype analysis conducted by carrying out a sWGS in hiPSCs-cl1. C CNV profile obtained by sWGS in hiPSCs-c.5483G > A. D CNV profile obtained by sWGS in hiPSCs-cl1. E GUIDE-seq analysis of gRNA + 4. F Sanger sequence of the top one predicted off-target of gRNA + 4 in hiPSCs-cl1. The protospacer is highlighted in black, PAM in red