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. 2022 Sep 2;13:443. doi: 10.1186/s13287-022-03062-0

Fig. 1.

Fig. 1

Downregulation of CDC20 in the process of adipogenic differentiation of hBMSCs. A CDC20 mRNA expression at day 0, 4, 7, 10, and 14 during the process of adipogenic differentiation of hBMSCs examined by qRT-PCR. (B-D) PPARG B, CEBPA C, and FABP4 D mRNA expression levels at day 0, 4, 7, 10, and 14 during the process of adipogenic differentiation of hBMSCs examined by qRT-PCR. (E, F) Western blotting analysis E and quantification F of CDC20, PPARγ, C/EBPα, FABP4 and the internal control GAPDH protein levels in proliferation medium (PM) and adipogenic medium (AM) for 7 days. All data are presented as the mean ± SD (n = 3, **P < 0.01, ***P < 0.001)