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. 2022 Sep 2;13:443. doi: 10.1186/s13287-022-03062-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Knockdown of CDC20 enhances adipogenic differentiation of hBMSCs. A Fluorescence micrographs showing the lentivirus transduction efficiency. Scale bar, 500 μm. B CDC20 mRNA expression in control shRNA (NC) and CDC20 knockdown (shCDC20-1, shCDC20-2) groups examined using qRT-PCR. (C, D) Oil red O staining C and quantification D of hBMSCs after adipogenic induction for 21 days. Scale bar, 100 μm. (E, F) Adipogenic regulators PPARG E and CEBPA F mRNA expression levels determined by qRT-PCR after adipogenic induction for 14 days. (G, H) Western blotting analysis G and quantification H of CDC20, PPARγ, C/EBPα, and the internal control GAPDH of negative control (NC) and CDC20 knockdown (shCDC20) cells in proliferation medium (PM) and adipogenic medium (AM) for 7 days. All data are presented as the mean ± SD (n = 3, **P < 0.01, ***P < 0.001)