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. 2022 Sep 2;13:443. doi: 10.1186/s13287-022-03062-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Conditional knockout of Cdc20 increases adipogenic differentiation of mBMSCs. (A, B) The knockout efficiency of Cdc20 in BMSCs of Cdc20^f/f and Sp7-Cre;Cdc20^f/f mice examined by qRT-PCR A and western blotting analysis B. (C, D) Oil red O staining C and quantification D at day 21 after adipogenic induction of mBMSCs from Cdc20^f/f and Sp7-Cre;Cdc20^f/f mice. Scale bar, 100 μm. (E, F) Adipogenic markers Pparg E and Cebpa F mRNA expressions determined by qRT-PCR after adipogenic induction for 14 days of mBMSCs from Cdc20^f/f and Sp7-Cre;Cdc20^f/f mice. All data are presented as the mean ± SD (n = 3, ***P < 0.001)