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. 2022 Aug 25;2022:9455152. doi: 10.1155/2022/9455152

Figure 3.

Figure 3

miR-3960 targets and negatively regulates PHLDA2. (a) The target gene of miR-3960 predicted using TargetScan, miRWalk, and upregulated genes in the OA-related GSE16464 dataset from GEO database. (b) PHLDA2 expression in GSE16464 dataset. (c) The binding sites between miR-3960 and PHLDA2 predicted using starBase. (d) PHLDA2 mRNA level in the cartilage tissues of OA patients (n = 20) and patients with traumatic amputation (n = 20) determined by RT-qPCR. (e) Correlation between miR-3960 and PHLDA2 analyzed by Pearson's correlation coefficient. (f) The binding of miR-3960 to PHLDA2 verified using the dual-luciferase reporter gene assay. Chondrocytes were transduced with miR-3960 mimic or miR-3960 inhibitor. (g) miR-3960 expression and PHLDA2 mRNA level in the chondrocytes determined by RT-qPCR. (h) PHLDA2 protein level in the chondrocytes determined by immunoblotting. ∗p < 0.05 vs. patients with traumatic amputation or chondrocytes treated with mimic-NC; #p < 0.05 vs. chondrocytes treated with IL-1β; & p < 0.05 vs. chondrocytes treated with inhibitor-NC. Data are shown as the mean ± standard deviation of three technical replicates. Data between two groups were compared by independent t-test. Data comparisons among multiple groups were analyzed by the one-way ANOVA with Tukey's post hoc test.