Table 1.
Antigen | Supplier | Product no. | Species | Dilution (WB) | Dilution (IF) |
---|---|---|---|---|---|
GLUT1 | Alpha Diagnostics | GT11-A | rabbit | 1:2000 | 1:250 |
GLUT3 | Alpha Diagnostics | GT31-A | rabbit | 1:1000 | 1:100 |
Rhodopsin | Santa Cruz | sc-57432 (1D4) | mouse | 1:1000 | 1:250 |
Rod arrestin (Arr1) | Philp Lab | rabbit | 1:1000 | not used | |
M-opsin | Millipore | ab5405 | rabbit | 1:1000 | 1:200 |
cone arrestin (Arr3) | EMD/Millipore | ab15282 | rabbit | 1:1000 | 1:1000 |
Iba-1 | Waco | 19-19741 | rabbit | not used | 1:100 |
Vinculin | Cell Signaling | 13901 | rabbit | 1:1000 | not used |
β-actin | Cell Signaling | 4970 | rabbit | 1:2000 | not used |
anti-mouse IgG- 488 | Invitrogen | A32766 | donkey | not used | 1:500 |
anti-mouse IgG- 555 | Invitrogen | A32773 | donkey | not used | 1:500 |
anti-rabbit IgG- 488 | Invitrogen | A32790 | donkey | not used | 1:500 |
anti-rabbit IgG- 555 | Invitrogen | A32794 | donkey | not used | 1:500 |
anti-rabbit IgG-HRP | Jackson Immuno Research | 111-035-144 | goat | 1:2000 | not used |
anti-mouse IgG-HRP | Jackson Immuno Research | 115-035-062 | goat | 1:2000 | not used |