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. Author manuscript; available in PMC: 2022 Sep 2.
Published in final edited form as: Cell Rep. 2022 Aug 2;40(5):111161. doi: 10.1016/j.celrep.2022.111161

Figure 2. Combined prenatal stressors induce lasting changes in offspring communication and social behavior.

Figure 2.

(A) Behavioral testing in offspring born to CON and DEP + MS dams.

(B) Representative spectrograms of USVs.

(C) MUPET syllable classification.

(D and E) USV number and total call time at P8 (n = 14–19 mice/condition/sex, two-way ANOVA with Holm-Sidak’s post hoc tests).

(F) Repertoire units were organized from shortest to longest and displayed as the absolute difference between group means.

(G and H) Schematic of three-chamber social preference test (n = 6–7 mice/condition/sex, two-way ANOVA with Sidak’s multiple comparisons post hoc tests).

(I and J) Schematic of three-chamber social novelty preference test. (n = 6–7 mice/condition/sex, two-way ANOVA, with Sidak’s multiple comparisons post hoc tests).

(K) Schematic of USV courtship assay.

(L and M) Adult USV syllable length and call time (n = 15–17 mice/condition, unpaired t-tests).

(N) Repertoire units organized from shortest to longest are displayed as the absolute difference between group means. Means ± SEM.