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. Author manuscript; available in PMC: 2022 Sep 2.
Published in final edited form as: Methods Mol Biol. 2022;2474:59–69. doi: 10.1007/978-1-0716-2213-1_7

Table 1:

Cell culture and assay medium for ARE-bla assay

Components Culture Medium Assay Medium Thaw Medium
DMEM 90% 99% 90%
Dialyzed FBS 10% 1% 10%
NEAA 0.1 mM 0.1 mM 0.1 mM
HEPES 25 mM 25 mM 25 mM
Penicillin 100 U/mL 100 U/mL 100 U/mL
Streptomycin 100 μg/mL 100 μg/mL 100 μg/mL
Blasticidin 5 μg/mL - -