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. 2022 Aug 19;13:975291. doi: 10.3389/fphar.2022.975291

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Copyright © 2022 Liu, Cao, Liu, Gao, Du, Wang, Cheng, Zhu, Jia, Pan, Zhang, Jiang, He, Xu, Zhang, An, Guo and Gu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Preparation and characterization of T7-siYY1-exo. (A) Schematic diagram of preparation and isolation of T7-siYY1-exo. (B) Detection of CD9, CD63, TSG101, Lamp2b, HA, and GM130 expression by Western blot. (C) Detection of YY1 knockdown efficiency. (D) Size distribution of T7-exo and T7-siYY1-exo measured by NANO SIGHT. (E)Transmission electron micrograph of T7-exo and T7-siYY1-exo. (F) qRT-PCR analysis of siYY1-Cy3 after treatments with RNase A/T1 Mix and 1% Triton X-100 for 30 min.