Fig 1. Acsl1 expression is upregulated in macrophages by HG.

A) BMDMs were differentiated under normal glucose (NG) and high glucose (HG), and Acsl1 mRNA absolute copy number was determined by quantitative real-time PCR (qPCR) using a standard curve generated with known quantities of mouse Acsl1 cDNA. B) Western blot of total cell lysates from BMDMs cultured in NG and HG using antibodies against ACSL1 and β-actin as (loading control). C) The ratio of ACSL1/β-actin band intensities was quantified using Image studio 5.2 (n = 3). D) Nascent Acsl1 RNA expression in BMDMs under NG and HG conditions was determined by qPCR. Nascent Acsl1 expression was quantified relative to cyclophilin A and shown as fold change. The data presented are the means ± standard error (n = 3); the p-value was calculated using the student’s t-test. (*p < 0.05).