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. 2022 Sep 2;17(9):e0272986. doi: 10.1371/journal.pone.0272986

Fig 2. CHREBP contributes to transcriptional upregulation of Acsl1 in HG.

Fig 2

A) CHREBP expression plasmid or vector only (VO) were co-transfected with Acsl1-GLuc reporter in HEK293 cells cultured under HG conditions. Luciferase assay was performed 48 hours post-transfection and shown as relative luciferase units (RLU). Expression of transfected Myc-tagged CHREBP is shown by western blot using a c-Myc antibody, with tubulin as a loading control. B) Wild type or Chrebp-/- BMDMs were differentiated under HG conditions. Acsl1 mRNA absolute copy number was quantified by qPCR. The data presented are means ± standard error (n = 3); the p-value was calculated using student’s t-test. (*p < 0.05).