Skip to main content
. 2022 Sep 2;17(9):e0272986. doi: 10.1371/journal.pone.0272986

Fig 5. CHREBP and NF-kappa B increase Acsl1 transcriptional activity.

Fig 5

HEK293 cells cultured in HG conditions were transfected with Acsl1-GLuc reporter (250ng; columns 1–7) and individually with p65/RELA (150ng; column 2) or CHREBP (150ng; column 3). Cells were transfected with Acsl1-GLuc reporter and a fixed amount of p65/RELA (150ng; columns 4–7), along with increasing amounts of CHREBP (100ng, 150ng, 200ng, 250ng; columns 4–7, respectively). Total DNA was adjusted to 750ng with vector only. Luciferase assay was performed 48 hours post-transfection and shown as relative luciferase units (RLU). The data presented are means ± standard errors (n = 3); the p-value was calculated using one-way ANOVA. *p < 0.05. B) Western blot of lysates from panel A with antibodies against the Myc-tag on CHREBP, FLAG-tag on p65/RELA, and tubulin as a loading control. The p65/RELA blot for lanes 1–2 was run on a different gel than lanes 3–7 and denoted by a black line between lanes 2 and 3. Exposure times were the same.