Table 1.

Classification of EVs.

Type of EVs	Size (nm)/Shape	Biogenesis	Cargo content
Small EVs/Exosomes	30-100nm, Spheroid	They carrying ILVs are generated by ESCRT machinery and merge with the plasma membrane for release. Bascially early endosomes mature into late endosomes	HSPs, Tetraspanins (CD9, CD63, CD81), Biogenesis components (ALIX, TSG101) and integrins.
Large EVs/Microvesicles	100-1000, irregular	Membrane outward budding, followed by fission via contractile machinery	Integrins, selectins, CD40 ligand, fotillin-2, Death receptors (CD40) adenosine diphosphate ribosylation factor 6, phosphatidylserine, VCAMP3, ARF6
Exomeres	~50nm, spheroid	non-membranous nanoparticle isolated from the small EVs	High concentration of metabolic enzymes and hallmark related proteins in glycolysis and mTORC1 signalling
Apoptotic bodies	>1000, variable	During planned cell death, cytoplasmic fragmentation occurs.	Annexin V, Caspase 3, Phosphatidylserine and histones.
Oncosomes	>1000	Cell body cleavage to large cytoplasmic extensions	Cell adhesion molecules (CD44, integrins, ICAM), cytokeratine 18, CD9, CD81.