Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Mouse monoclonal | Developmental Studies Hybridoma Bank (DSHB) |
AB_528269 | 1:50 |
| Antibody | Mouse monoclonal | DSHB | AB_2314866 | 1:100 |
| Antibody | Mouse monoclonal | DSHB | AB_528203 | 1:100 |
| Antibody | Mouse monoclonal | DSHB | AB_2617422 | 1:500 |
| Antibody | Rabbit polyclonal | Pielage et al., 2005 | 1:10000 | |
| Antibody | Rabbit polyclonal | Perry et al., 2017 | 1:1000 | |
| Antibody | Guinea polyclonal | Goel and Dickman, 2018 | 1:2000 | |
| Antibody | Guinea polyclonal | Perry et al., 2017 | 1:1000 | |
| Antibody | Alexa Fluor 647 conjugated Goat anti-Horseradish Peroxidase |
Jackson ImmunoResearch Laboratories (Jackson) |
123-605-021 | 1:400 |
| Antibody | Alexa Fluor 488 conjugated secondary antibodies |
Jackson | 706-545-148, 715-545-150, 711-545-152 |
1:400 |
| Antibody | Cy3-conjugated secondary antibodies |
Jackson | 706-165-148, 715-165-150, 711-165-152 |
1:400 |
| Antibody | DyLight 405-conjugated secondary antibodies |
Jackson | 706-475-148, 715-475-150 | 1:400 |
| Antibody | Alexa Fluor 647 conjugated Goat anti-Phalloidin |
ThermoFisher | A22287 | 1:1000 |
| Genetic reagent (D. melanogaster) | UAS-BoNT-C | This study | See Materials and methods, subsection Molecular biology. |
|
| Genetic reagent (D. melanogaster) | UAS-BoNT-A | This study | Same as above | |
| Genetic reagent (D. melanogaster) | UAS-BoNT-B | This study | Same as above | |
| Genetic reagent (D. melanogaster) | UAS-BoNT-E | This study | Same as above | |
| Genetic reagent (D. melanogaster) | MHC-CD8-GCaMP8f-Sh (SynapGCaMP8f) |
This study | Same as above | |
| Genetic reagent (D. melanogaster) | OK6-GAL4 | Aberle et al., 2002 | ||
| Genetic reagent (D. melanogaster) | UAS-rpr.hid | Zhou et al., 1997 | ||
| Genetic reagent (D. melanogaster) | OK319-GAL4 | Sweeney et al., 1995 | ||
| Genetic reagent (D. melanogaster) | vGlutSS1 | Sherer et al., 2020 | ||
| Genetic reagent (D. melanogaster) | B3RT-vGlut-B3RT | Sherer et al., 2020 | ||
| Genetic reagent (D. melanogaster) | UAS-B3 | Sherer et al., 2020 | ||
| Genetic reagent (D. melanogaster) | UAS-ChR2T159C | Bloomington Drosophila
Stock Center (BDSC) |
58373 | |
| Genetic reagent (D. melanogaster) | UAS-CD4::tdGFP | BDSC | 35839 | |
| Genetic reagent (D. melanogaster) | dHb9-GAL4 | BDSC | 83004 | |
| Genetic reagent (D. melanogaster) | GMR27E09-GAL4 | BDSC | 49227 | |
| Genetic reagent (D. melanogaster) | UAS-TNT | BDSC | 28838 | |
| Genetic reagent (D. melanogaster) | w1118 | BDSC | 5905 | |
| Genetic reagent (D. melanogaster) | D42-GAL4 | BDSC | 8816 | |
| Recombinant DNA reagent | pUASt destination vector |
Drosophila Genetics Resource Center (DGRC) |
1129 | |
| Chemical, compound, drug | all trans-Retinal | Sigma-Aldrich | R2500 | |
| Software | NIS Elements software | Nikon | 4.51.01 | |
| Software | Axon pCLAMP Clampfit | Molecular Devices | 10.7 | |
| Software | MiniAnalysis | Synaptosoft | 6.0.3 | |
| Software | GraphPad Prism | GraphPad | 8.0.1 | |
| Software | Jupyter Notebook | Anaconda | 6.0.1 | |
| Software | ImageJ (Fiji) | Rueden et al., 2017 |