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. 2022 Aug 12;11:e77848. doi: 10.7554/eLife.77848

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© 2022, Cheng et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Author response image 2. — (A) t-SNE plot of cell clusters with the filtering criteria of nFeature_RNA (200-6500) in the single-cell analysis by Seurat. (B) t-SNE clustering as in (A) but colored by genotype. (C) Left panel: cell type composition and percentage as colored in (A). Right panel: percentage of cell clusters of the oligodendrocyte lineage. (D) Heatmap showing the expression levels of cell-type signature genes. (E) FeaturePlot of the representative genes (Mal and Nkx6-2) specifically suppressed in the late stages of OLs in Stag2-depleted forebrains. A maximum cutoff of 3 was used.