Figure 3.

miR-613 increased NPC cell radiosensitivity by inhibiting DNMT3B. (a) mRNA levels of DNMT3B in cancer tissues and adjacent tissues of 121 NPC patients were detected by RT-qPCR. (b) Correlation between DNMT3B and miR-613. (c) Binding relationship between DNMT3B and miR-613 was verified by dual-luciferase assay. (d–e) Protein level of DNMT3B in cells was detected by Western blot. (f) Cell viability of C666-1R cells was detected using the CCK-8 method. (g) Cell clone formation and apoptosis in C666-1R cells were detected by colony formation and (h) flow cytometry. Three repeated tests were performed. Data were expressed as mean ± SD, and data in panels (a) and (c) were analyzed using independent t test; data in panels (d), (e), (g), and (h) were analyzed using one-way ANOVA, followed by Tukey's test; and data in panel (f) were analyzed using repeated variance test. ∗p < 0.05, ∗∗p < 0.01.