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. 2022 Jul 29;41(36):4231–4243. doi: 10.1038/s41388-022-02413-8

Fig. 6. LVBU promotes tumorigenesis in vivo.

Fig. 6

a–c Dox-inducible HCT116-shLVBU cells were subcutaneously injected into nude mice. Mice was i.p. injected with PBS or Dox (to induce LVBU knockdown). Tumor growth curves and final tumor weight are shown (N = 6 independent tumors, *p < 0.05). d The mRNA expression of LVBU and BCL6 were detected by qRT-PCR in tumor tissues obtained from (a). (N = 6 independent samples, ***p < 0.001). e The mRNA expression of indicated urea cycle genes was detected by qRT-PCR in sh-NC or sh-LVBU tumor tissues obtained from (a). (sh-NC, negative control. N = 3 independent samples, *p < 0.05; **p < 0.01; ***p < 0.001). f Representative IHC staining of Ki-67, BCL6, p53, ARG1, ODC1 and OTC in tumor tissues obtained from (a). The staining intensity of indicated proteins in (f) was quantitated by Image J and presented as bar graphs (N = 3 independent tumors, *p < 0.05; **p < 0.01; ***p < 0.001). g Relative cell proliferation rate was examined after cells were transfected with LVBU overexpression plasmids with or without DFMO treatment (N = 3 independent samples, *p < 0.05; ***p < 0.001). h, i Dox-inducible HCT116-shLVBU cells were subcutaneously injected into nude mice. Mice were i.p. injected with PBS or Dox or DOX + DFMO. Tumor growth curves and final tumor weight are shown (N = 4 independent tumors, *p < 0.05; **p < 0.01).