Table 3.
CA/09-like virus-infection induced focused HAI antibody responses.
| Patient | Sera | HAIa | Fold reductionb | Focused HAI antibody responses | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CA/09 | MX/09 | X-179A | RG | RG | RG | X-179A | RG | RG | RG | ||||
| No. (#) | dpo† | dpicu† | wt | Q223QR | Q223R | K163Q | 127gly | 130del | Q223R | K163Q | 127gly | 130del | |
| #1* | 8 | 2 | 2560 | 40 | < | 1280 | 2560 | 80 | 256 | –‡ | – | 32 | K130, Q223 |
| 14 | 8 | 160 | 40 | 40 | < | 160 | 160 | 4 | 16 | – | – | K163, Q223 | |
| 28 | 22 | 2560 | 2560 | 640 | 160 | 2560 | 2560 | 4 | 16 | – | – | K163, Q223 | |
| #2* | 3 | 3 | 2560 | 160 | 80 | 2560 | 2560 | 160 | 32 | – | – | 16 | K130, Q223 |
| 4 | 4 | 2560 | 160 | 80 | 2560 | 2560 | 160 | 32 | – | – | 16 | K130, Q223 | |
| #3 | 18 | 1 | 1280 | 1280 | 1280 | 1280 | 1280 | 160 | – | – | – | 8 | K130 |
| 35 | 18 | 1280 | 1280 | 1280 | 1280 | 1280 | 160 | – | – | – | 8 | K130 | |
| 41 | 24 | 1280 | 1280 | 1280 | 1280 | 1280 | 160 | – | – | – | 8 | K130 | |
| #4 | 13 | 7 | 640 | 320 | 640 | 320 | 40 | 40 | – | – | 16 | 16 | 127gly, K130 |
| 16 | 10 | 1280 | 1280 | 1280 | 1280 | 160 | 160 | – | – | 8 | 8 | 127gly, K130 | |
| 28 | 22 | 1280 | 1280 | 1280 | 1280 | 160 | 160 | – | – | 8 | 8 | 127gly, K130 | |
| #5 | 18 | 2 | 320 | 320 | 320 | 320 | 80 | 160 | – | – | 4 | – | 127gly |
| 45 | 29 | 640 | 640 | 640 | 640 | 80 | 160 | – | – | 8 | 4 | 127gly, K130 | |
| #6 | 8 | 1 | 320 | 320 | 80 | 320 | 320 | 80 | 4 | – | – | 4 | K130, Q223 |
| 11 | 4 | 1280 | 640 | 320 | 1280 | 1280 | 320 | 4 | – | – | 4 | K130, Q223 | |
| 14 | 7 | 2560 | 2560 | 640 | 1280 | 1280 | 640 | 4 | – | – | 4 | K130, Q223 | |
| #7 | 3 | 2 | 320 | 160 | 160 | 320 | 160 | 320 | – | – | – | – | ND¥ |
| 22 | 21 | 12,800 | 12,800 | 12,800 | 12,800 | 12,800 | 12,800 | – | – | – | – | ND | |
| #8 | 9 | 2 | 160 | 160 | 80 | 40 | 80 | 160 | – | 4 | – | – | K163 |
| 14 | 7 | 5120 | 2560 | 5120 | 1280 | 5120 | 5120 | – | 4 | – | – | K163 | |
| #9 | 13 | 2 | 80 | 80 | 40 | 80 | 80 | 40 | – | – | – | – | ND |
| 15 | 4 | 320 | 320 | 320 | 320 | 320 | 160 | – | – | – | – | ND | |
| #10 | 9 | 2 | 80 | 80 | 80 | 80 | < | < | – | – | 8 | 8 | 127gly, K130 |
| 14 | 7 | 640 | 1280 | 1280 | 640 | 80 | 80 | – | – | 8 | 8 | 127gly, K130 | |
| 18 | 11 | 1280 | 1280 | 1280 | 1280 | 160 | 160 | – | – | 8 | 8 | 127gly, K130 | |
| #11* | 9 | 2 | 80 | 80 | 80 | 80 | 80 | < | – | – | – | 16 | K130 |
| #12 | 5 | 1 | 80 | 40 | 40 | 80 | 20 | 80 | – | – | 4 | – | 127gly |
| 7 | 3 | 160 | 160 | 80 | 320 | 40 | 320 | – | – | 4 | – | 127gly | |
| 14 | 10 | 640 | 320 | 160 | 640 | 80 | 640 | 4 | – | 8 | – | 127gly, Q223 | |
| #13 | 25 | 5 | 80 | 80 | 80 | 20 | 80 | 80 | – | 4 | – | – | K163 |
| 30 | 10 | 320 | 320 | 320 | 40 | 320 | 320 | – | 8 | – | – | K163 | |
| 40 | 20 | 640 | 640 | 640 | 80 | 640 | 640 | – | 8 | – | – | K163 | |
| #14 | 2 | 2 | 80 | 40 | 20 | 80 | 80 | 20 | 4 | – | – | 4 | K130, Q223 |
| 4 | 4 | 1280 | 160 | 20 | 1280 | 1280 | 40 | 64 | – | – | 32 | K130, Q223 | |
| 10 | 10 | 1280 | 80 | 40 | 1280 | 1280 | 40 | 32 | – | – | 32 | K130, Q223 | |
| #15 | 13 | 8 | 320 | 320 | 320 | 320 | 80 | 160 | – | – | 4 | – | 127gly |
| 27 | 22 | 640 | 640 | 640 | 640 | 80 | 160 | – | – | 8 | 4 | 127gly, K130 | |
| #16 | 8 | 3 | 320 | 160 | 160 | 160 | 160 | 160 | – | – | – | – | ND |
| 12 | 7 | 640 | 640 | 640 | 640 | 640 | 640 | – | – | – | – | ND | |
| #17 | 9 | 5 | 80 | 80 | 80 | 80 | 40 | 20 | – | – | – | 4 | K130 |
| 11 | 7 | 320 | 320 | 320 | 320 | 160 | 40 | – | – | – | 8 | K130 | |
| 19 | 15 | 320 | 320 | 320 | 320 | 160 | 40 | – | – | – | 8 | K130 | |
| #18 | 8 | 4 | 160 | 160 | 160 | 160 | 160 | 160 | – | – | – | – | ND |
| 34 | 30 | 2560 | 1280 | 1280 | 1280 | 1280 | 1280 | – | – | – | – | ND | |
| #22 | 20 | 6 | 320 | 320 | 160 | 160 | 160 | 320 | – | – | – | – | ND |
| 36 | 22 | 640 | 640 | 640 | 640 | 320 | 640 | – | – | – | – | ND | |
| #23 | 15 | 7 | 640 | 640 | 320 | 640 | 80 | 640 | – | – | 8 | – | 127gly |
| 22 | 14 | 1280 | 1280 | 1280 | 1280 | 160 | 1280 | – | – | 8 | – | 127gly | |
| 29 | 21 | 1280 | 1280 | 1280 | 1280 | 320 | 1280 | – | – | 4 | – | 127gly | |
| #24 | 11 | 4 | 160 | 80 | 160 | 160 | 40 | 20 | – | – | 4 | 8 | 127gly, K130 |
| 13 | 6 | 640 | 1280 | 640 | 640 | 160 | 40 | – | – | 4 | 16 | 127gly, K130 | |
| 27 | 20 | 2560 | 2560 | 2560 | 2560 | 640 | 160 | – | – | 4 | 16 | 127gly, K130 | |
| #25* | 13 | 7 | 5120 | 640 | 320 | 5120 | 5120 | 640 | 16 | – | – | 8 | K130, Q223 |
| #26 | 12 | 6 | 80 | 160 | 160 | 160 | 160 | 80 | – | – | – | – | ND |
| 21 | 15 | 640 | 640 | 640 | 640 | 640 | 320 | – | – | – | – | ND | |
| #27 | 7 | 6 | 160 | 160 | 160 | 20 | 160 | 160 | – | 8 | – | – | K163 |
| 23 | 22 | 320 | 160 | 320 | 40 | 320 | 320 | – | 8 | – | – | K163 | |
| #29 | 10 | 7 | 320 | 320 | 160 | 80 | 160 | 160 | – | 4 | – | – | K163 |
| 16 | 13 | 1280 | 1280 | 640 | 320 | 640 | 640 | – | 4 | – | – | K163 | |
| #31 | 17 | 14 | 320 | 320 | 320 | 320 | 160 | 160 | – | – | – | – | ND |
| 24 | 21 | 320 | 320 | 320 | 320 | 160 | 160 | – | – | – | – | ND | |
†dpo, days post-symptom onset; †dpicu, days post ICU admission; ‡–, ≤ twofold reductions; ¥ND, not detected.
*Fatal patients in bold. For each sample, we completed three independent HAI assays.
aSera from 26 patients possessing HAI titers of ≥ 80 against wt-CA/09 were tested by HAI assays with the 6 viruses.
bFold reduction of HAI titers against virus-mutant(s) compared to wt-CA/09.