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. 2022 Sep 2;13:5172. doi: 10.1038/s41467-022-32912-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Images of the TBI cavity before and after implanting the CMH scaffold (left panel). Images of CMH injected into a star-shaped mold via a syringe (right panel). b Schematic of the in vivo experimental timeline, including the sacrifice and analysis time points. c Fluorescence images of Iba-1 immunostaining after treatment with PBS, MB, CMH, and CMH + HFMF. d Analysis of Iba-1, scar thickness and NF200-positive responses at peri-trauma areas (n = 6, mean ± s.d., one-way ANOVA with Tukey’s multiple-comparison test). Fluorescence images of (e) GFAP and (f) NF200 immunostaining after various treatments. g Analysis of the NF200-positive responses and infiltration distance in the trauma area. (n = 6, mean ± s.d., one-way ANOVA with Tukey’s multiple-comparison test).