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. 2022 Sep 2;13:5161. doi: 10.1038/s41467-022-32759-2

Fig. 2. Proton binding closes the intracellular side of HnSpns.

Fig. 2

a Spin label pairs (blue spheres) for DEER distance measurement of the gating helices across the NTD and CTD on the intracellular side of the IF crystal structure and the intracellular-closed (IC) model. b Raw DEER decays and fits (left) are presented for the experimentally-determined distributions P(r) in nanodiscs (middle) and LMNG micelles (right) for two distance pairs, M84-S386 and S148-F324 (dotted lines in a), under acidic, neutral, and basic pH conditions. Confidence bands (2σ) are shown about the best fit lines. This band, which depicts the estimated uncertainty in P(r), reflects the error associated with the fitting of the primary DEER trace. The distributions predicted from the IF crystal structure and the IC model are shaded gray and red, respectively.