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. 2022 Sep 2;13:5159. doi: 10.1038/s41467-022-32829-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Promoter sequences were forward engineered with desired transcription rates and start sites. B Sequence analysis of the 75 bp forward engineered promoters showed low sequence similarity. C Model predictions are compared to in vivo transcription rate measurements for the 35 designed promoters (R² = 0.80, Spearman’s ρ = 0.89). Red dots denote the mean of duplicate measurements (n = 2 biological replicates). Data are provided in Supplementary Data 1.