Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 2;13:5159. doi: 10.1038/s41467-022-32829-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — A The predicted σ⁷⁰ transcriptional profile of an 11-promoter genetic circuit⁸ is compared to in vivo transcription rate and start site measurements on the (top) sense and (bottom) anti-sense strand. Transcription flux ratios are the measured differences in adjacent mRNA levels from RNA-Seq. White and gray shadows correspond to each transcribed cistron. The horizontal dotted black lines show the minimum transcription rates that define a start site. Experimental TSS cutoffs are previously described⁸, and prediction cutoffs are defined in the Methods. Annotated start sites are depicted in the circuit diagram with arrows. B The predicted σ⁷⁰ transcriptional profile for the P_BAD-amtR portion of the circuit in the OFF state. Red lines are model predictions, and gray overlays show measured RNAP flux. C The predicted σ⁷⁰ transcriptional profile for a redesigned amtR protein coding sequence that minimized the transcription initiation rate inside the coding sequence. (inset) Measured fluorescence levels for designed no-promoter regions predicted to have minimal transcription rates (NP1: 120-bp, NP2: 60-bp) as compared to (WC) white cells and the J23100 promoter. Black dots denote independent measurements. Bars denote the mean across duplicate measurements (n = 2 biological replicates). Data are provided in Supplementary Data 1.