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. 2022 Aug 30;54(8):1290–1305. doi: 10.1038/s12276-022-00837-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a MiR-375 overexpression in DU145 and PC-3 cells was verified via qRT-PCR after these cells were stably transfected with pSUPER-RETRO-Puro-miR-375 recombinant vector or pSUPER-RETRO-Puro-empty vector (serving as a control). CCK-8 and EdU incorporation assays were performed to assess the cell proliferation (b) and DNA synthesis rate (c) in miR-375-overexpressing DU145 and PC-3 cells. Transwell (d) and wound healing assays (e) were performed to confirm the effects of miR-375 overexpression on cell migration and invasion. f Flow cytometric analysis of the apoptotic rate in DU145 and PC-3 cells with or without miR-375 overexpression. g, h The protein levels of EMT and apoptosis markers in miR-375-overexpressing or miR-375-knockdown DU145 and PC-3 cells compared with the respective control cells. Cl. 1: Cell colony 1 transfected with pSUPER-RETRO-Puro-miR-375 recombinant vector. Cl. 2: Cell colony 2 transfected with pSUPER-RETRO-Puro-miR-375 recombinant vector. Vector: cell colony transfected with pSUPER-RETRO-Puro-empty vector (served as control). sp Cl.1: cell colony 1 transfected with pHB-U6-MCS-PGK-PURO-miR-375 sponge vector. Sp Cl.2: cell colony 2 transfected with pHB-U6-MCS-PGK-PURO-miR-375 sponge vector. NC: cell colony transfected with pHB-U6-MCS-PGK-PURO-empty vector (served as control).