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. 2022 Aug 30;54(8):1290–1305. doi: 10.1038/s12276-022-00837-6

Fig. 6. E-375i inhibited PCa proliferation, migration, and invasion while promoting apoptosis in DU145 cells.

Fig. 6

qRT-PCR illustrating miR-375 AMO loaded and intercellular transferred by hucMSC derived exosomes inhibited expression of miR-375 in DU145 and PC-3 cells (a), diminished the inhibitory effect on PTPN4 (b) in a dose-dependent manner determined by qRT-PCR, as well as downregulated the protein expression of p-STAT3 and upregulated PTPN4 (c) in a dose-dependent manner in DU145 cells, which were determined by western blot. d EdU incorporation and e CCK-8 assays were used to assess the role of miR-375 AMO-loaded exosomes on the proliferation of DU145 cells. f Transwell and g wound-healing assays were applied to test the effects of miR-375 AMO on cell migration and invasion in DU145 cells. h Flow cytometry was conducted to test the function of miR-375 AMO on the apoptotic rate of DU145 cells. i The effect of miR-375 AMO on the protein expression of p-STAT3, EMT and apoptosis markers in DU145 cells. PBS: cells treated with PBS that served as suspension control of exosomes. NC: scramble oligonucleotide. exo: exosomes suspended in PBS. e-NC: exosomes loaded with scramble oligonucleotides. e-375i: exosomes loaded with miR-375 AMO.