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. 2022 Aug 30;54(8):1290–1305. doi: 10.1038/s12276-022-00837-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — AR protein (a) and mRNA (b) levels in BPH and PCa cell lines. c The protein levels of AR in miR-375-overexpressing or miR-375-knockdown DU145 and PC-3 cells compared with the control cells. Western blotting was conducted to evaluate AR expression in DU145 and PC-3 cells in response to d overexpression of miR-375 and/or PTPN4 and e suppression of miR-375 and/or PTPN4. f The effect of miR-375 AMO on the protein expression of AR in DU145 and PC-3 cells. The mRNA expression of AR in DU145 and PC-3 cells was verified via qRT-PCR after miR-375 was overexpressed (g) or knocked down (h). i The correlation between miR-375 levels and AR in PCa, statistically processed by StarBase. r = 0.096, p = 3.28e-2. j The expression of miR-375 and k AR in PCa tissues compared with paired normal prostate tissues from TCGA database. The mRNA levels of AR in PTPN4- and/or miR-375-overexpressing (l) and PTPN4- and/or miR-375-suppressed (m) DU145 and PC-3 cells, determined by qRT-PCR. n qRT-PCR results showing the transcription levels of AR in DU145 and PC-3 cells after treatment with e-375i or the controls. o Western blot showing the expression of AR in DU145 tumor tissues as the expression of miR-375 was manipulated. p IHC analysis of AR expression in DU145 tumor tissues as miR-375 expression was manipulated. q Western blotting showing the expression of AR in tumor tissues treated with e-375i. r IHC analysis of the expression of AR in the e-375i and e-NC groups. Scramble: cells stably transfected with pHB-U6-MCS-PGK-PURO-empty vector and transiently transfected with siNC. SiPTPN4: cells stably transfected with pHB-U6-MCS-PGK-PURO-empty vector and transiently transfected with siPTPN4 vector. Sp miR-375: cells stably transfected with pHB-U6-MCS-PGK-PURO-miR-375 sponge and transiently transfected with siNC. Sp miR-375 + siPTPN4: cells stably transfected with pHB-U6-MCS-PGK-PURO-miR-375 recombinant vector and transiently transfected with siPTPN4.