REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
STAT6 rabbit monoclonal (C-terminal -targeted)	Abcam	Cat#ab32520; RRID:AB_778113
LAMP1 rabbit polyclonal	Abcam	Cat#ab24170; RRID:AB_775978
BiP rabbit polyclonal	Abcam	Cat#ab21685; RRID:AB_2119834
Catalase rabbit polyclonal	Abcam	Cat#ab52477; RRID:AB_868694
HSP90AB1 mouse monoclonal	Abcam	Cat#ab53497; RRID:AB_881097
Phospho-PKC alpha (S657) rabbit monoclonal	Abcam	Cat#ab180848; RRID:AB_2783796
Myc rabbit monoclonal	Cell signaling	Cat#2278; RRID:AB_490778
Myc mouse monoclonal	Cell signaling	Cat#2276; RRID:AB_331783
FLAG rabbit monoclonal	Cell signaling	Cat#14793; RRID:AB_2572291
FLAG mouse monoclonal	Cell signaling	Cat#8146; RRID:AB_10950495
STAT1 rabbit polyclonal	Cell signaling	Cat#9172; RRID:AB_2198300
STAT2 rabbit polyclonal	Cell signaling	Cat#4594; RRID:AB_2271323
STAT3 rabbit monoclonal	Cell signaling	Cat#12640; RRID:AB_2629499
STAT5 rabbit monoclonal	Cell signaling	Cat#94205; RRID:AB_2737403
STAT6 rabbit monoclonal (SH2 -targeted)	Cell signaling	Cat#5397; RRID:AB_11220421
MFN2 rabbit monoclonal	Cell signaling	Cat#11925; RRID:AB_2750893
COXⅣ rabbit monoclonal	Cell signaling	Cat#4850; RRID:AB_2085424
Phospho-JNK rabbit monoclonal	Cell signaling	Cat#4668; RRID:AB_823588
JNK rabbit monoclonal	Cell signaling	Cat#9258; RRID:AB_2141027
Phospho-ERK rabbit monoclonal	Cell signaling	Cat#4370; RRID:AB_2315112
ERK rabbit monoclonal	Cell signaling	Cat#4695; RRID:AB_390779
Phospho-p38 rabbit monoclonal	Cell signaling	Cat#4511; RRID:AB_2139682
P38 rabbit polyclonal	Cell signaling	Cat#9212; RRID:AB_330713
Phospho-CREB rabbit monoclonal	Cell signaling	Cat#9198: RRID:AB_2561044
Phospho-NFkB rabbit monoclonal	Cell signaling	Cat#3033; RRID:AB_331284
PKC alpha rabbit polyclonal	Cell signaling	Cat#2056; RRID:AB_2284227
STAT6 rabbit polyclonal (280-480 a.a.-targeted)	SantaCruz	Cat#sc-1698; RRID:AB_2197235
TOM20 rabbit polyclonal	SantaCruz	Cat#sc-11415; RRID:AB_2207533
TOM40 rabbit polyclonal	SantaCruz	Cat#sc-11414; RRID:AB_793274
Aconitase 2 rabbit polyclonal	SantaCruz	Cat#sc-130677; RRID:AB_2221514
GAPDH goat polyclonal	SantaCruz	Cat#sc-48167; RRID:AB_1563046
Alpha-tubulin mouse monoclonal	SantaCruz	Cat#sc-5286; RRID:AB_628411
Actin mouse monoclonal	SantaCruz	Cat#sc-47778; RRID:AB_626632
Cytochrome c mouse monoclonal	SantaCruz	Cat#sc-13156; RRID:AB_627385
TIM23 mouse monoclonal	SantaCruz	Cat#sc-514463; RRID:AB_2923126
Calnexin mouse monoclonal	SantaCruz	Cat#sc-46669; RRID:AB_626784
Alexa Fluor Plus 488, anti-Mouse	Invitrogen	Cat#A32766; RRID:AB_2762823
Alexa Fluor Plus 488, anti-Rabbit	Invitrogen	Cat#A32790; RRID:AB_2762833
Alexa Fluor 546, anti-Rabbit	Invitrogen	Cat#A10040; RRID:AB_2534016
Bacterial and virus strains
E.Coli. (DH5alpha)	RBC	Cat#RH617
E.Coli. (Stbl3)	invitrogen	Cat#C737303
Chemicals, peptides, and recombinant proteins
DMEM	WELGENE	Cat#LM 001-05
Trypsin-EDTA	WELGENE	Cat#LS 015-10
Antibiotic-antimycotic solution	Gibco	Cat#15240-062
PBS	Gibco	Cat#21600-010
FBS, premium	GenDEPOT	Cat#F0600-050
Protease inhibitor cocktail	GenDEPOT	Cat#P3100-005
G418	Sigma-Aldrich	Cat#A1720
Puromycin	Sigma-Aldrich	Cat#P8833
Polyethylene glycol (PEG)	Sigma-Aldrich	Cat#P7181
Cycloheximide solution	Sigma-Aldrich	Cat#C4859
Paraformaldehyde	Sigma-Aldrich	Cat#P6148
Saponin	Sigma-Aldrich	Cat#47036
PMSF	Sigma-Aldrich	Cat#10837091001
TCA	Sigma-Aldrich	Cat#T8657
Percoll	Sigma-Aldrich	Cat#P1644
Duolink-PLA	Sigma-Aldrich	Cat#DUO92007
Crystal violet solution	Sigma-Aldrich	Cat#V5265
Nitro blue tetrazolium	Sigma-Aldrich	Cat#N5514
Agarose, low-gelling temperature	Sigma-Aldrich	Cat#A9045
Lipofectamine3000	Invitrogen	Cat#L3000015
TMRM	Invitrogen	Cat#T668
Protein G Dynabeads	Invitrogen	Cat#10003D
mitochondria-GFP, BacMam 2.0	Invitrogen	Cat#C10508
mitochondria-RFP, BacMam 2.0	Invitrogen	Cat#C10505
DSP	Thermo Scientific	Cat#A35393
Proteinase K	BIONEER	Cat#KB-0111
Mounting medium (with DAPI)	Vector Laboratories	Cat#H-1200
6nm colloidal gold particle, anti-Rabbit	Jackson ImmunoResearch	Cat#711-195-152
Critical commercial assays
ATP assay	Abcam	Cat#ab83355
OCR assay	Cayman	Cat#600800
Deposited data
Raw and analyzed data	This paper	Mendeley Data: https://doi.org/10.17632/44fxxng896.6
Experimental models: Cell lines
293T	ATCC	Cat#CRL-3216; RRID:CVCL_0063
HeLa	Korean Cell Line Bank	Cat#10002; RRID:CVCL_0030
B16-F10	Korean Cell Line Bank	Cat#80008; RRID:CVCL_0159
Hep3B	Korean Cell Line Bank	Cat#88064; RRID:CVCL_0326
HEK293	Korean Cell Line Bank	Cat#21573; RRID:CVCL_0045
T98-MG	Korean Cell Line Bank	Cat#21690; RRID:CVCL_0556
U373-MG	Korean Cell Line Bank	Cat#30017; RRID:CVCL_2219
U87-MG	Korean Cell Line Bank	Cat#30014; RRID:CVCL_0022
STAT6 WT –U373-MG	This paper	N/A
Δ 1-260 –U373-MG	This paper	N/A
Δ DBD –U373-MG	This paper	N/A
Δ LD –U373-MG	This paper	N/A
Δ SH2 –U373-MG	This paper	N/A
Δ TAD –U373-MG	This paper	N/A
Δ 1-50 –U373-MG	This paper	N/A
Δ 674-847 –U373-MG	This paper	N/A
Δ 699-847 –U373-MG	This paper	N/A
Δ 774-847 –U373-MG	This paper	N/A
MFN2-Myc/MFN2-Flag –293T	This paper	N/A
STAT6 KO –B16-F10	This paper	N/A
STAT6 KO –293T	This paper	N/A
STAT6 WT-Myc –293T (generated from STAT6 KO –293T)	This paper	N/A
Δ SH2-Myc –293T (generated from STAT6 KO –293T)	This paper	N/A
Δ MTS-Myc –293T (generated from STAT6 KO –293T)	This paper	N/A
Experimental models: Organisms/strains
C57BL/6J	OrientBio	N/A
BALB/c nude (CAnN.Cg-Foxn1nu/ CrljOri)	OrientBio	N/A
Oligonucleotides
See Table S2	See Table S2
Recombinant DNA
pCMV6-Entry (Myc-DDK-tagged)	ORIGENE	Cat#PS100001
STAT6 WT (Myc-DDK-tagged)	(Park et al., 2019)	N/A
Δ 1-260 (Myc-DDK-tagged)	(Park et al., 2019)	N/A
Δ DBD (Myc-DDK-tagged)	(Park et al., 2019)	N/A
Δ LD (Myc-DDK-tagged)	(Park et al., 2019)	N/A
Δ SH2 (Myc-DDK-tagged)	(Park et al., 2019)	N/A
Δ TAD (Myc-DDK-tagged)	(Park et al., 2019)	N/A
Δ 1-50 (Myc-DDK-tagged)	This paper	N/A
Δ 674-847 –U373-MG	This paper	N/A
Δ 699-847 (Myc-DDK-tagged)	This paper	N/A
Δ 774-847 (Myc-DDK-tagged)	This paper	N/A
Δ 31-260 (Myc-DDK-tagged)	This paper	N/A
pLenti-C-mGFP	ORIGENE	Cat#PS100071
pLenti-STAT6 WT-mGFP	ORIGENE	Cat#RC210065L2V
pLenti-Δ SH2-mGFP	This paper	N/A
pLenti-Δ MTS-mGFP	This paper	N/A
pCMV6-AC-GFP	ORIGENE	Cat#PS100010
31-50 (STAT6 N-terminal fragment)-GFP	This paper	N/A
1-30 (STAT6 N-terminal fragment)-GFP	This paper	N/A
1-20 (STAT6 N-terminal fragment)-GFP	This paper	N/A
16-25 (STAT6 N-terminal fragment)-GFP	This paper	N/A
16-30 (STAT6 N-terminal fragment)-GFP	This paper	N/A
21-30 (STAT6 N-terminal fragment)-GFP	This paper	N/A
11-30 (STAT6 N-terminal fragment)-GFP	This paper	N/A
pCMV6-AN-GFP	ORIGENE	Cat#PS100019
GFP-1-30	This paper	N/A
MFN2 WT (Myc-DDK-tagged)	ORIGENE	Cat#RC226143
Δ HR1 (Myc-DDK-tagged)	This paper	N/A
Δ PR (Myc-DDK-tagged)	This paper	N/A
Δ HR2 (Myc-DDK-tagged)	This paper	N/A
pLenti-C-Myc-DDK-IRES-Puro	ORIGENE	Cat#PS100069
pLenti-MFN2-Myc (-IRES-Puro)	This paper	N/A
pLenti-MFN2-Flag (-IRES-Puro)	This paper	N/A
pLenti-STAT6 WT (C-Myc-DDK-IRES-Puro)	This paper	N/A
pLenti-Δ SH2 (C-Myc-DDK-IRES-Puro)	This paper	N/A
pLenti-Δ MTS (C-Myc-DDK-IRES-Puro)	This paper	N/A
pLenti-C-mCherry	This paper	N/A
pLenti-STAT6 WT-mCherry	This paper	N/A
pLenti-Δ SH2- mCherry	This paper	N/A
pLenti-Δ MTS- mCherry	This paper	N/A
Mouse STAT6 CRISPR/Cas9 KO Plasmid	Santa Cruz	Cat#sc-423180
Human STAT6 CRISPR/Cas9 KO Plasmid	Santa Cruz	Cat#sc-418159
Software and algorithms
Image J	National Institutes of Health, USA	https://imagej.nih.gov/ij/
DAVID	The Laboratory of Immunopathogenesis and Bioinformatics	https://david.ncifcrf.gov/home.jsp
Prism 9.0	GraphPad	N/A
SPSS 25.0	IBM	N/A
membrane protein explorer (MPEx)	The Stephen White laboratory at UC Irvine	https://blanco.biomol.uci.edu/mpex/