Antibodies |
|
GR/NR3C1 Antibody (Rabbit polyclonal) |
Novus Biologicals |
Cat#NBP2-42221 |
normal rabbit IgG |
Santa Cruz Biotechnology |
Cat#sc-2027 |
Ki67 antibody (Mouse monoclonal) |
Santa Cruz Biotechnology |
Cat#sc-23900 |
Anti-Mouse IgG Secondary Antibody, Alexa Fluor 488 (Donkey polyclonal) |
ThermoFisher Scientific |
Cat#A2102 |
|
Chemicals, peptides, and recombinant proteins |
|
Hydrocortisone |
Sigma Aldrich |
Cat#H4001 |
DMSO |
Sigma Aldrich |
Cat#D8418 |
Relacorilant |
Corcept Therapeutics |
N/A |
DMEM |
Gibco |
Cat#31053-028 |
Fetal bovine serum (FBS) |
VWR Life Science Seradigm |
Cat#97068-085 |
L-glutamine |
Thermo Fisher Scientific |
Cat#25-030-081 |
Sodium pyruvate |
Gibco |
Cat#11360-070 |
Non-essential amino acids |
Gibco |
Cat#11140-050 |
Antibiotic-Antimycotic |
Gibco |
Cat#15240-062 |
ezDNase |
ThermoFisher Scientific |
Cat#11766051 |
|
Critical commercial assays |
|
Dual-Luciferase Reporter Assay System |
Promega |
Cat#E1910 |
GenFind V3 DNA extraction and purification kit |
Beckman Coulter |
Cat#C34880
|
RNeasy Micro Kit |
QIAGEN |
Cat#74004 |
Super-Script IV VILO Master Mix |
ThermoFisher Scientific |
Cat#11756050 |
EZ DNA Methylation Kit |
Zymo Research |
Cat#D5001 |
Collibri PCR-free DNA Library Prep Kit |
ThermoFisher Scientific |
Cat#A38609024 |
|
Deposited data |
|
Sequencing and microarray data |
Gene Expression Omnibus (GEO) |
GEO: GSE210304
|
|
Experimental models: Cell lines |
|
IMR-90 lung fibroblasts |
Coriell Institute for Medical Research |
Cat#I90-10 |
|
Software and algorithms |
|
ImageJ |
National Institutes of Health |
https://imagej.nih.gov/ij/ |
R 3.6.3 |
R Core Team |
https://www.r-project.org/ |