Fig. 6.

Oxidized phospholipids accumulates in alkoxyl radicals-treated chondrocytes and suppress bone development. (A) Lipid peroxidation level was detected by flow cytometry after incubating with C11-BODIPY in ATDC5 cells. (B) Normal phase LC/MS chromatogram (grey) and mass spectra of PLs in ATDC5 cells. (C) OxPLs in chondrocytes were extracted and analyzed by principal component analysis, and the 2D score plots display repertoires of control and AAPH-treated embryos. Each point represents a sample, and ellipses represent 95% confidence regions (n = 4). (D–E) Heat map and Volcano plot showing differentially expressed OxPLs between control group and AAPH group. Each dot represents one class of phospholipids and the fold change was set to >2.0. (F) Expressions of chondrogenesis-related genes were measured by qRT-PCR. ATDC5 cells were treated with SAPE-OOH (2 μM). Data are presented as mean ± SD. Comparisons between groups were made using one-way ANOVA (A) and Student's t-test (F). **P < 0.01, ***P < 0.001 vs the Control group. ^###P < 0.001 vs the AAPH-treated group. ^$$P < 0.01 vs the Erastin-treated group.