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. 2022 Aug 2;298(9):102324. doi: 10.1016/j.jbc.2022.102324

Figure 1.

Figure 1

Maternal HC diet causes poor embryonic skeletal development in C57BL6/J mice. A, effect of C and HC diet on maternal gestational weight gain and (B) litter size. C, effect of maternal C and HC diet on newborn offspring’s body weight and (D) body length at birth. Representative alizarin red and alcian blue–stained images of (E) whole mount and skull ((F) dorsal side, (G) ventral side, (H) lateral side, and (I) mandible) of C and HC group embryo at E 18.5 days. J, fold change in skull length over C. Representative alizarin red and alcian blue–stained (K) sternum and (L) fore limb images. Fold change in length of (M) scapula and humerus over C. Representative alizarin red and alcian blue–stained (N) hind limb images. Fold change in (O) osteoid index and femur length over C. Representative alizarin red and alcian blue–stained images of (P) forepaw and (Q) hind paw. Scale bar as indicated in the images. Each group contains ≥6 animals. For alcian blue/alizarin red staining studies four litters with a minimum of three animals per litter were analyzed. Data are represented as mean±SEM (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001). C, control; HC, high cholesterol.