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. 2022 Aug 2;298(9):102324. doi: 10.1016/j.jbc.2022.102324

Figure 3.

Figure 3

Maternal HC diet causes reduced embryonic skeletal development in Swiss Albino mice. A, effect of C and HC diet on maternal gestational weight gain and (B) litter size. (C) effect of maternal C and HC diet on newborn offspring’s body weight and (D) body length at birth. Representative alizarin red and alcian blue–stained images of (E) whole mount and skull ((F) dorsal side, (G) ventral side, (H) lateral side, and (I) mandible) of C and HC group embryo at E 18.5 days. (J) fold change in skull length over C. Representative alizarin red and alcian blue–stained images of (K) sternum and fold change in (L) length of sternum over C. (M) representative alizarin red and alcian blue–stained images of the forelimb. Fold change in (N) osteoid index and length of scapula and humerus over C. (O) representative alizarin red and alcian blue–stained images of Hind limb. Fold change in (P) osteoid index and length of femur and tibia over control. Representative alizarin red and alcian blue–stained images of (Q) forepaw and (R) hind paw. Scale bar as indicated in the images. Each group contains ≥6 animals. Data are represented as mean ± SEM (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001). C, control; HC, high cholesterol.