Table 1.
Oligos used for strain construction.
| Oligo | Name | Sequence |
|---|---|---|
| P1 | LeAF1 | ACAGGTACTTCCATCTTGTACTGGT |
| P2 | LeAF2 | TCtcctccaacgtccgatct |
| P3 | LeAR3 | acctccactagctccagcaagccgaacagaggtaaagacga |
| P4 | hphF4 | tcgtctttacctctgttcggcttgctggagctagtggaggtca |
| P5 | hphR5 | taccaacgtgcgaccatttTCTcggtcggcatctactctattcct |
| P6 | LeAF6 | aggaatagagtagatgccgaccgagaaaatggtcgcacgttggta |
| P7 | LeAR7 | AAGCGTCTCTTTCCTGGGTCTT |
| P8 | LeAR8 | TGCCAGTTCTGTTGGACATCTCT |
| P15 | gpdAF | cgcagatctcaagctgtaaggatttcggca |
| P16 | gpdAR | CACCGGGCCCATCTCAAACATTGTGATGTCTGCTCAAGCG |
| P17 | laeAF | cgcttgagcagacatcacaatgtttgagatgggcccggtg |
| P18 | laeAR | cgcagatctGAGGATTATGAGAAGGGAGC |
| P19 | pyrG5F | GTAACGCCAGGGTTTTCCCAGTCACGACGtttaaacATGCATCATTCTCCCGCTTTGT |
| P20 | pyrG3R | tgccgaaatccttacagcttgAAGCTTcatcgccaatcacctcaatcac |
| P21 | LaeA5F | gtgattgaggtgattggcgatgAAGCTTcaagctgtaaggatttcggca |
| P22 | LaeA3R | acttctacacagccatcggtccAAGCTTgaggattatgagaagggagct |
| P23 | trc5F | agctcccttctcataatcctcAAGCTTggaccgatggctgtgtagaagt |
| P24 | trp3R | cgtaatcaattgcccgtctgtcagagagcggattcctcagtctcgt |
| P25 | PTR5F | acgagactgaggaatccgctctctgacagacgggcaattgattacg |
| P26 | PTR3R | acagcagtgcttatctgcgatgacgagccgctcttgcatctttgt |
| P27 | pyrG5F | acaaagatgcaagagcggctcgtcatcgcagataagcactgctgt |
| P28 | pyrG3R | GCGGATAACAATTTCACACAGGAAACAGCgtttaaactgtgccagtcaattgtccgaagt |
| P29 | upstF | cgaggtcgacggtatcgataGTTTAAACCTCCCAGGTACCGACTAAC |
| P30 | upstR | ctcaatcacaGATCATGTTTGGGTGGGTTC |
| P31 | ElaeAF | aaacatgatcTGTGATTGAGGTGATTGGCG |
| P32 | ElaeAR | ctctgtgcctACAGCAGTGCTTATCTGCGATG |
| P33 | downT | gcactgctgtAGGCACAGAGTAACAGGTAGGTAGACAG |
| P34 | downR | agtggatcccccgggctgcaGTTTAAACTCCCACGCACGAAAGCAACT |
| P107 | hygR | GTACTTCTACACAGCCATCGGTCCA |
| P108 | hygL | CGTTATGTTTATCGGCACTTTGCAT |