Fig 9.

Summary of the response of B. distachyon to inoculation with H. seropedicae (HS) in two nitrogen conditions. At 0.5 mM NH₄NO₃ HS causes increase of leaf area, elongation of primary root, and shortening of root hairs. Inoculated plants also depleted NH₄⁺ faster from the medium. Their nitrate uptake genes NRT2.1 (both time points) and NRT1.1 (28 DAT) in shoot have higher expression, corresponding to behavior at low NO₃⁻. At 5 mM NH₄NO₃ inoculated plants show longer primary roots and shorter root hairs than non-inoculated plants. They deplete less NO₃⁻ from the medium. Yellow stars indicate significant differences by non-invasive phenotyping. Relative expression, as ratio of inoculated versus non-inoculated is shown in the heat maps, with high (dark blue) and low ratio (white); asterisks indicate significant t-test in the inoculated versus non-inoculated plants. N-form uptake (gray triangles): increased depletion from medium (upright traingle), decreased depletion (inverted triangle). Control, non-inoculated; +HS, inoculated plants; LR, lateral roots; PR, primary root; RH, root hairs.