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. 2022 Sep 3;21:175. doi: 10.1186/s12943-022-01643-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — BAF complex disruption reduces collagen adhesion and invasion in vitro, and causes morphological changes and destruction of stress fibres in neuroblastoma cells. A Adhesion assays of neuroblastoma cells to collagen-coated 96-well plates. Cells were aspirated and rinsed at the indicated time points after seeding. Attached cells were quantified using crystal violet staining and absorbance values were normalized to non-aspirated cells. B Invasion assay with collagen-coated transwells. Neuroblastoma cells (2 × 10⁵ cells/well) were seeded in collagen-coated transwells and let migrate for 16 h. Invaded cells were quantified by crystal violet staining C Representative microscopy pictures invaded cells. D Representative images of neuroblastoma cells stained with phalloidin (F-Actin), anti-tubulin antibody and DAPI by confocal microscopy. E Quantification of tubulin-free phalloidin area per cell of SK-N-BE(2) (upper graph) and SH-SY5Y (lower graph) cell lines. Ten fields per biological replicate n = 3 were analyzed. ** means p < 0.01; *** means p < 0.001