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. 2022 Sep 1;219(11):e20211861. doi: 10.1084/jem.20211861

Figure 1.

Figure 1.

BATF deficiency in ILCs causes spontaneous colitis. (A) Batffl/fl (WT, n = 10) and littermate control Batffl/flPLZF-cre (cKO, n = 18) mice were monitored for incidence of rectal prolapse. (B and C) Representative images of the colon (B) from WT and littermate control cKO mice aged 12 wk old and quantification of clone length (C). (D and E) Representative images of the spleen and mLN (D) from WT and littermate control cKO mice aged 12 wk as well as quantification of spleen weight (E). (F and G) H&E staining of ileum and colon sections (F) from WT and littermate control cKO mice aged 12 wk and quantification of histological scores (G). Scale bar, 100 µm. (H and I) Frequency of total activated (CD44+CD62L−) and naive (CD44−CD62L+) CD4+ T cells from the SI (H) and LI (I) of WT and littermate control cKO mice aged 12 wk. (J) Representative flow plots of intracellular staining of IL-17A, IFN-γ, and TNF-α in SI CD4+ T cells after stimulation with PMA plus ionomycin ex vivo from WT and littermate control cKO mice aged 12 wk. (K) Quantification of plots in J. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed unpaired t test). Each dot represents one mouse, n = 4–6 mice per group. Data are pooled from two (C, E, and G) or three (H and I) experiments or are representative of at least two independent experiments.