Figure S1.

BATF deficiency in ILCs causes spontaneous colitis. Related to Fig. 1. (A) Flow cytometry histograms analyzing the expression of BATF in the SI ILCs (Lin⁻CD90.2⁺) and ILC3s (Lin⁻CD90.2⁺RORγt⁺) of WT and cKO mice. (B) Representative image of rectal prolapse of cKO (right) and littermate control WT (left) mice aged 12 wk. (C) Flow cytometry analysis of the frequency of total neutrophils (CD11b⁺Ly6G⁺) in the SI and LI of cKO and littermate control WT mice aged 12 wk. (D and E) Quantified frequencies of neutrophils in the SI (D) and LI (E) as assessed in C. (F) Flow cytometry analysis of frequency of total macrophages (CD11b⁺F4/80⁺) in the SI and LI of cKO and littermate control WT mice aged 12 wk. (G and H) Quantified frequencies of neutrophils in the SI (G) and LI (H) as assessed in C. (I and J) Quantified cell number of naive and effector CD4⁺ T cells in the SI (I) and LI (J) as assessed in C. Data shown as the mean ± SEM. ***P < 0.001 (two-tailed unpaired t test). Each dot represents one mouse, n = 4–6 mice per group. Data are pooled from two independent experiments.